CyTOF Interest Group Meeting

The next CyTOF Interest Group meeting is scheduled for Tuesday, October 11, from 1:15 to 2:15 pm in BJCIH 7 A/B conference room. 

 

presentation by

Josh Alinger (French Lab) 

Paper discussion:

"Highly multiplexed simultaneous detection of RNAs and proteins in single cells"

 

Additional Information

Josh Alinger will present a recent paper describing PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry.

http://www.nature.com/nmeth/journal/v13/n3/full/nmeth.3742.html

Frei AP, Bava FA, Zunder ER, Hsieh EW, Chen SY, Nolan GP, Gherardini PF. Highly multiplexed simultaneous detection of RNAs and proteins in single cells. Nat Methods. 2016 Mar;13(3):269-75. doi: 10.1038/nmeth.3742. Epub 2016 Jan 25.

Abstract:
To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each analyzed cell defined on the basis of the expression of a separate set of transcripts or proteins. By expanding high-throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.

 

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Fall 2016 Schedule

Tues 9/13 - Melissa Berrien-Elliott (Fehniger Lab)
Tues 10/11 - Josh Alinger  (French Lab)
Tues 11/8 - Jeff Atkinson lab
Tues 12/13 - Tony French lab