IML Core Lab

About

The Immunomonitoring Laboratory (IML) was established in March 2014 within The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs (CHiiPs) through funding by the Chancellor of Washington University in St. Louis, the Dean of the School of Medicine and Chairs of Medicine, Pathology and Immunology, Radiation Oncology and Surgery/Head of the Siteman Cancer Center.

Overview

The IML provides the structure, instrumentation and expertise to monitor the impact of specific immunotherapies on human and mouse immunologic profiles, interrogate mechanisms and pathways related to treatment protocols and advance the conceptual basis for targeted treatment strategies. The IML works closely with the Tissue Procurement Center (TPC) to access clinical samples.

Primary Objectives

  • To conduct assays and provide expertise to support efforts in the areas of human and mouse immunology and immunotherapy (bench to bedside).
  • To explore new basic mechanisms derived from analysis of immune mediated diseases and immunotherapy (bedside to bench).
  • To synergize with other Washington University Centers and Programs to investigate novel roles of immunity in disease processes.
  • To harmonize assay procedures across Immunomonitoring shared resources.

Services

  • Produce custom human and murine MHC I tetramers 
  • Conjugate mAbs and tetramers with metal-containing polymer tags or fluorophores
  • Provide single and multiple cytokine analyses from serum, plasma, cell culture supernatants or other fluids
  • Perform lymphocyte functional assays
  • Promote identification of new biomarkers for translational research
  • Develop barcoding panels for immunophenotype analysis of patient samples
  • Identify unique cellular components/subsets and map interrelated signaling networks
  • Track distinct cellular populations in samples following identification by barcoding
  • Provide tetramer staining and instrumentation support for Immunoassay laboratory

Equipment

Cytometry

  AKTA Pure FPLC              Mass Cytometry CyTOF2                   Fortessa     
Tetramer Production
AKTA Pure FPLC
  Mass Cytometry
CyTOF2
  Flow Cytometry
Fortessa X20 Cytometer
         

Immunoassays

Flow Cytometry                                        ELISPOT                                 BioTek Synergy H1 Plate Reader                 
Meso Scale Discovery
Quickplex-120                               
  CTL-Immunospot S6
Universal Analyzer (ELISPOT)
  BioTek Synergy H1 Plate Reader
(absorbance, fluorescence and
luminescence) BioTek Plate Washer
         

 Click here to contact a member of the IML Team.